Spectrophotometric determination of dropropizine in pure form and phrmaceutical formulation

Three new simple, different and sensitive spectrophotometric methods are suggested for the determination of Dropropizine [DRO]. The first is based on the reduction of ferric into ferrous in presence of 0-phenanthroline by the mentioned drug to form a highly stable orange-red ferroin chelate[Fe-[Phen][3]][2+], measured at [lambda][max] 510.5 nm for zero-order [D[degree sign]] while the peak amplitude was measured at 537.5 the nm for the first derivative [D[1]] using distilled deionized water as a solvent. Beer's law was obeyed in the range of 0.5-2.2 micro g/ml for both D[degree sign] and D[1] with mean percentage recoveries of 100.08 +/- 1.370 and 100.03 +/- 1.064, respectively. The second method is based on the reaction of DRO with 7,7,8,8- tetracyanoquinodimethane [TCNQ] in acetonitrile solvent to form a highly stable charge transfer colored complex that was measured at 734.5 nm. Beer's law was obeyed in the range of 10-55 micro g/ml with mean percentage recovery of 99.93 +/- 1.070. The last method is based on the reaction of DRO with cerium [IV] ammonium sulphate in acidic medium to form a highly stable colored oxidative product that was measured at 486.5 nm. Beer's law was obeyed in a concentration range 145 - 335 micro g/ml with mean percentage recovery of 99.67 +/- 1.077. The optimum assay conditions and their applicability for the determination of the cited drug in pharmaceutical formulation were described. The proposed methods were statistically compared with the reported method revealing good accuracy and precision

Stability - indicating methods for the determination of lorazepam, delorazepam and tetrazepam

Two simple, rapid and accurate techniques have been developed for the determination of lorazepam, delorazepam and tetrazepam in the presence of their degradation products. The first one depends on the derivative ratio spectrophotometry while the second depends on spectrodensitometry. The validity of the proposed procedures was proved using standard addition technique and laboratory-prepared mixtures of the drugs and their degradation products. The proposed procedures were successfully applied for the drugs analysis in their tablet dosage form

Spectrofluorimetric determination of bisoprolol fumarate and valsartan in tablets and spiked plasma

Simple, sensitive and rapid spectrofluorimetric procedure is described for the determination of two antihypertensive drugs namely, Bisoprolol fumarate [BSF] and Valsartan [VT]. The effect of solvents was investigated. The fluorescence properties of the two cited drugs showed maximum emission intensity in 0.1N H[2]SO[4] at lambda [em] 298 and 415nm for BSF and VT, respectively, when excited at lambda[ex] 227nm. The calibration graphs were rectilinear from 0.08-1.28 and 0.12-1.6 micro g/ml for BSF and VT respectively. The method, was applied to the determination of the two cited drugs in tablets either single or when co-formulated with hydrochlorothiazide [HZ] with % recoveries of 100.03 +/- 0.57 and 99.70 +/- 0.90 for BSF and VT, respectively. Furthermore, the high sensitivity of the proposed method it allowed its application in spiked human plasma with good % recoveries of 99.73 +/- 2.06 for BSF and 99.94 +/- 1.71 for VT

Worries and concerns of diabetic pregnant women

Women may experience a variety of worries and concerns in associations with pregnancy. Pregnancy in diabetic women makes these worries and concerns endless and intensified. Gathering information about those worries and concerns during pregnancy is required to contribute to the provision of adequate and effectiveness of psychological care that might help in coping more effectively with pregnancy time for diabetic women. This study is concerned with identifying worries and concerns of diabetic pregnant women and assessing their feelings. It was conducted at the out patient obstetrics clinics of three hospitals namely EI-Mainshawy Health Insurance Hospital, Mahala General Hospital, and EI-Aiada El-Shamela in Tanta. The target population of this study consisted of 400 pregnant women. Among the subjects, 200 were diabetic and 200 nondiabetic pregnant women as a control group. Data were collected through three tools: structured worries and concerns questionnaire, feelings word checklist, and Taylor Manifest Anxiety Scale. The results of this study yielded that diabetic pregnant women expressed significantly higher negative emotions [fear, anxiety, angry, and sadness] and mixed [happiness and sadness] than nondiabetic [chi[2]=52.370 and 14.148, respectively]. Nondiabetic women had significant higher free anxiety level score than diabetic [chi[2]=28.711]. Among diabetic women, 33% had severe anxiety level. Concern for baby was the highest worry rated by the diabetic women [93%] as compared to 43.5% of nondiabetic women. All diabetic women [100%] were concerned by fear of baby might have diabetes, increase dose of medication, and caesarean section. Applying pregnancy counselling involving all issues specific to the diabetic patient when planning for pregnancy was the main recommendation from this study

Three different techniques for the analysis of chlordesmethyldiazepam

Three simple, rapid and accurate different techniques have been developed for the determination of chlordesmethyldiazepam. The first one depends on the spectrophotometric determination of the orange azodye resulting from the coupling of thymol with the acid-induced hydrolysis product of chlordesmethyldiazepam after conversion to the corresponding diazonium salt in alkaline medium. The second is the application of quantitative densitometry for the determination of chlordesmethyldiazepam in the presence of its degradation benzophenone product. While, the third technique implies HPLC resolution efficiency in the determination of chlordesmethyldiazepam in the determination of chlordesmethyldiazepam in the presence of its degradation benzophenone product

Stability indicating methods for the determination of bromazepam

Three simple, accurate and stability indicating methods were developed for the determination of bromazepam in the presence of its degradation product. The first method depends on measuring the peak height of the first derivative difference absorbance after complete acid hydrolysis at 420 nm. The second method utilizes densitometry after separation on precoated TLC plates, while the third implies HPLC using RP 18 column, acetonitrile/water [40:60] as a mobile phase and diode array detector

Spectrophotometric determination of midazolam using 2,3-dichloro-5,6-dicyano-P-benzoquinone

In the present work, substituted quinines such as 2,3-dichloro-5,6- dicyano-p-benzoquinone [DDQ] is utilized in spectrophotometric determination of midazolam through the formation of orange-red colored charge transfer complex. The color is characterized by a maximum absorbance at 462.5 nm. Different reaction parameters were investigated. The proposed procedure was successfully applied for the determination of midazolam in bulk powder over a concentration range of 66-330 mug ml-1 and in different pharmaceutical preparations

Spectrophotometric determination of penicillamine by indirect method

Spectrophotometric determination of penicillamine was achieved through its oxidation to penicillaminic acid using known excess of freshly prepared hypobromite, then residual hypobromite was treated with sodium flourescein in buffered medium at pH 5.57, where equivalent eosin is produced which absorbs maximally at 517 nm. Stiochiometric study of the reaction showed that, penicillamine reacts with hypobromite in the ratio of 1:3 respectively. Concentrations adhering to Beer's law are from 40-110 micro g%. The method was applied to artamin capsules and its validity was ascertained by standard addition technique. The mean percentage recovery +/- S.D was found to be 99.37 +/- 0.58. The proposed method is sensitive, accurate and precise as compared with official method

Alkaloids and lignans of haplophyllum tuberculatum

The aerial parts of Haplophyllum tuberculatum [Forssk.] A Juss. Grown in Egypt yielded the furoquinoline alkaloids: dihydroperfamine [I], and skimmianine, [II]; the aryenaphthalene lignans: justicidin-A [III] and B [IV]. Skimmianine and justicidin-B were identified by direct comparison with authentic references, while dihydroperfamine and justicidin-A were identified by analysis of their spectral data. Dihydroperfamine showed direct relaxation on smooth muscles, as well as a hypotensive action